Publications

2021

Anna M. Duraj-Thatte*, Avinash Manjula-Basavanna*, Jarod Rutledge, Jing Xia, Shabir Hassan, Arjirios Sourlis, Andrés G. Rubio, Ami Lesha, Michael Zenkl, Anton Kan, David A. Weitz, Yu Shrike Zhang, Neel S. Joshi (2021). Programmable Microbial Ink for 3D Printing of Living Materials Produced from Genetically Engineered Protein Nanofibers. Nature Communications 12:6600, DOI: 10.1038/s41467-021-26791-x
Duraj-Thatte, A. M., Manjula-Basavanna, A., Courchesne, N. M. D., Cannici, G. I., Sánchez-Ferrer, A., Frank, B. P., van’t Hag, L., Cotts, S. L., Fairbrother, D. H., Mezzenga, R., & Joshi, N. S. (2021). Water-processable, biodegradable and coatable aquaplastic from engineered biofilms. Nat Chem Biol, DOI: 10.1038/s41589-021-00773-y [Author final draft].
Manjula-Basavanna, A., Duraj-Thatte, A. M., & Joshi, N. S. (2021). Robust Self‐Regeneratable Stiff Living Materials Fabricated from Microbial Cells. Adv. Funct. Mater. 2010784.
Kan, A., Gelfat, I., Emani, S., Praveschotinunt, P., & Joshi, N. S. (2021). Plasmid Vectors for in Vivo Selection-Free Use with the Probiotic E. coli Nissle 1917. ACS Synthetic Biology, DOI: 10.1021/acssynbio.0c00466.

2020

Manjula-Basavanna, A., Duraj-Thatte, A. M., & Joshi, N. S. (2020). Robust Self-Regeneratable Stiff Living Materials. bioRxiv 2020.09.24.311589.
Birnbaum, D. P., Manjula-Basavanna, A., Kan, A., & Joshi, N. S. (2020). Hybrid Living Capsules Autonomously Produced by Engineered Bacteria. bioRxiv 2020.11.23.394965.

2019

Praveschotinunt, P., Duraj-Thatte, A. M., Gelfat, I., Bahl, F., Chou, D. B., & Joshi, N. S. (2019). Engineered E. coli Nissle 1917 for the delivery of matrix-tethered therapeutic domains to the gut. Nature Communications, 10(1), 1-14.
Duraj‐Thatte, A. M., Courchesne, N. M. D., Praveschotinunt, P., Rutledge, J., Lee, Y., Karp, J. M., & Joshi, N. S. (2019). Genetically Programmable Self‐Regenerating Bacterial Hydrogels. Advanced Materials, 31(40), 1901826.
Kan, A., Birnbaum, D. P., Praveschotinunt, P., & Joshi, N. S. (2019). Congo Red fluorescence for rapid in situ characterization of synthetic curli systems. Applied and environmental microbiology, 85(13), e00434-19.
Kan, A., & Joshi, N. S. (2019). Towards the directed evolution of protein materials. MRS communications, 9(2), 441-455.

2018

Guo, J., Suástegui, M., Sakimoto, K. K., Moody, V. M., Xiao, G., Nocera, D. G., & Joshi, N. S. (2018). Light-driven fine chemical production in yeast biohybrids. Science, 362(6416), 813-816.
Courchesne, N. M. D., DeBenedictis, E. P., Tresback, J., Kim, J. J., Duraj-Thatte, A., Zanuy, D., … & Joshi, N. S. (2018). Biomimetic engineering of conductive curli protein films. Nanotechnology, 29(45), 454002.
Praveschotinunt, P., Dorval Courchesne, N. M., den Hartog, I., Lu, C., Kim, J. J., Nguyen, P. Q., & Joshi, N. S. (2018). Tracking of engineered bacteria in vivo using nonstandard amino acid incorporation. ACS synthetic biology, 7(6), 1640-1650.
Axpe, E., Duraj-Thatte, A., Chang, Y., Kaimaki, D. M., Sanchez-Sanchez, A., Caliskan, H. B., … & Joshi, N. S. (2018). Fabrication of amyloid curli fibers–alginate nanocomposite hydrogels with enhanced stiffness. ACS Biomaterials Science & Engineering, 4(6), 2100-2105.
Nguyen, P. Q., Courchesne, N. M. D., Duraj‐Thatte, A., Praveschotinunt, P., & Joshi, N. S. (2018). Engineered living materials: prospects and challenges for using biological systems to direct the assembly of smart materials. Advanced Materials, 30(19), 1704847.
Xiao, G., Chen, W., Tian, F., Richardson, J. J., Tardy, B. L., Liu, M., Joshi, N. S., & Guo, J. (2018). Thermal Transition of Bimetallic Metal–Phenolic Networks to Biomass‐Derived Hierarchically Porous Nanofibers. Chemistry–An Asian Journal, 13(8), 972-976.
Duraj-Thatte, A. M., Praveschotinunt, P., Nash, T. R., Ward, F. R., & Joshi, N. S. (2018). Modulating bacterial and gut mucosal interactions with engineered biofilm matrix proteins. Scientific reports, 8(1), 1-8.
Tay, P. K. R., Manjula-Basavanna, A., & Joshi, N. S. (2018). Repurposing bacterial extracellular matrix for selective and differential abstraction of rare earth elements. Green chemistry, 20(15), 3512-3520.

2017

Nussbaumer, M. G., Nguyen, P. Q., Tay, P. K., Naydich, A., Hysi, E., Botyanszki, Z., & Joshi, N. S. (2017). Bootstrapped Biocatalysis: Biofilm‐Derived Materials as Reversibly Functionalizable Multienzyme Surfaces. ChemCatChem, 9(23), 4328-4333.
Tay, P. K. R., Nguyen, P. Q., & Joshi, N. S. (2017). A synthetic circuit for mercury bioremediation using self-assembling functional amyloids. ACS synthetic biology, 6(10), 1841-1850.
Dorval Courchesne, N. M., Duraj-Thatte, A., Tay, P. K. R., Nguyen, P. Q., & Joshi, N. S. (2017). Scalable production of genetically engineered nanofibrous macroscopic materials via filtration. ACS Biomaterials Science & Engineering, 3(5), 733-741.

2016

Pardee, K., Slomovic, S., Nguyen, P. Q., Lee, J. W., Donghia, N., Burrill, …, Joshi, N. S., & Collins, J. J. (2016). Portable, on-demand biomolecular manufacturing. Cell, 167(1), 248-259.
Koshy, S. T., Desai, R. M., Joly, P., Li, J., Bagrodia, R. K., Lewin, S. A., Joshi, N. S., & Mooney, D. J. (2016). Click‐Crosslinked Injectable Gelatin Hydrogels. Advanced healthcare materials, 5(5), 541-547.
Dorval Courchesne, N., Tay, P. R., Nguyen, P. Q., & Joshi, N. S. (2016). Engineering E. coli to enable electron transfer along curli protein nanofibers. In Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf. FBIOE (Vol. 1972).

2015

Rubin, D., Joshi, N., & Desai, R. (2015). Peptide Nanotube Reinforced Polymers: A System for Tunable, Composite Materials. Harvard Medical School Boston United States.
Botyanszki, Z., Tay, P. K. R., Nguyen, P. Q., Nussbaumer, M. G., & Joshi, N. S. (2015). Engineered catalytic biofilms: Site‐specific enzyme immobilization onto E. coli curli nanofibers. Biotechnology and bioengineering, 112(10), 2016-2024.
Desai, R. M., Koshy, S. T., Hilderbrand, S. A., Mooney, D. J., & Joshi, N. S. (2015). Versatile click alginate hydrogels crosslinked via tetrazine–norbornene chemistry. Biomaterials, 50, 30-37.
Brudno, Y., Desai, R. M., Kwee, B. J., Joshi, N. S., Aizenberg, M., & Mooney, D. J. (2015). In vivo targeting through click chemistry. ChemMedChem, 10(4), 617-620.
Rubin, D. J., Amini, S., Zhou, F., Su, H., Miserez, A., & Joshi, N. S. (2015). Structural, nanomechanical, and computational characterization of D, L-cyclic peptide assemblies. ACS nano, 9(3), 3360-3368.

2014 and older

Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5(1), 1-10.
Rubin, D. J., Nia, H. T., Desire, T., Nguyen, P. Q., Gevelber, M., Ortiz, C., & Joshi, N. S. (2013). Mechanical reinforcement of polymeric fibers through peptide nanotube incorporation. Biomacromolecules, 14(10), 3370-3375.
Meister, G. E., & Joshi, N. S. (2013). An Engineered Calmodulin‐Based Allosteric Switch for Peptide Biosensing. ChemBioChem, 14(12), 1460-1467.